Project Documentation & Protocols
One of the goals of MaizeGDB is to collect documenation and protocols
from significant maize studies. We are collecting these
documents here as a central repository for future usage.
Below is a list of projects for which we archive documentation and
The B73 genome sequencing project
The maize genome sequencing project selected the cultivar B73 to be the
representative maize genome. The project published the first draft assembly of
the maize genome in 2009 (Schnable
et al., 2009).
Dooner and Du Ds-GFP insertions
This project produced a set of sequence-indexed stocks taged with transposed
Ds elements tagged with GFP. Some of these insertions have been validated by
the Fowler lab through a related project,
part of the Maize Gametophyte Project.
Maize Diversity Project (Panzea)
The Maize Diversity Project investigates the relationship between phenotype
and genotype with a focus on rare genetic variations. Information about this
project can be found at the Panzea website.
The NAM founders sequencing project
It has become very apparent that a significant portion of the genetic
diversity in maize is not captured in the representative genome assemblies of
B73. To capture a more complete pan-maize genome, a project to produce
assemblies of the highly diverse 25 maize NAM founders, all constructed with
the same technologies and processes was started in 2018. The complete genome
assemblies of all 25 founders were released in January, 2020.
UniformMu Mus2Use Transposon Resource
The latest set of UniformMu germinal insertions mapped in the maize
genome now available at MaizeGDB, and the corresponding consensus W22
insertion flanking sequences are deposited in Genbank. Individual
seed stocks containing these insertions are now available for
distribution through the Stock Center.
Cytogenetic Map of Maize Project
The Cytogenetic Map of Maize Project aims to produce a cytogenetic map
of the entire maize genome (NSF-DBI-0321639) using sorghum BACs as
fluorescence in situ hybridization (FISH) probes on pachytene
spreads. The goals of the project all involve using FISH to determine
the cytogenetic locations of genetically mapped maize marker
sequences. Specific aims are (1) to develop a framework cytogenetic
map with markers (CBMs) distributed at 10-20 cM intervals, (2) to
develop a detailed map of maize chromosome 9, and (3) to map
centromeric loci as an indirect method of locating the centromeres
more precisely within the current linkage maps.
Gene Discovery Project
In 1998, with a $12.5 million National Science Foundation grant, the
Maize Gene Discovery Project (MGDP) set out to identify a majority of
maize (Zea mays) genes in five years' time. They also planned
to develop mutant seeds, databases, and various tools that could be
used to determine the function of many maize genes. With that
foundation established, they hoped researchers could more efficiently
pursue studies that would improve crop yields, contribute to our
understanding of cereal genetics, and promote fundamental discoveries
in plant biology.
Maize Mapping Project
The primary goal of this project is to take the first step toward
understanding the structure and function of the Maize Genome by
developing and disseminating a comprehensive integrated physical
and genetic map of the Maize genome. This will involve the
preparation of Maize Genome fragments inserted into a Bacterial
Artificial Chromosome library, and the development and use of an
array of markers (Expressed Sequence Tags, Single Sequence Repeats
and Radiation Hybrids) to help determine the map. In all cases the
results (the map) and the reagents (the markers) will be made
available to the scientific community at large.